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Objective:To explore the significance of next-generation sequencing to detect gene mutations for diagnosis of patients with unexplained pancytopenia.Methods:The next-generation sequencing was used to detect 32 common hematological tumor gene mutations in 113 patients with unexplained pancytopenia in Huazhong University of Science and Technology Union Shenzhen Hospital from February 2016 to February 2019.Results:The next-generation sequencing results showed that of the 32 genes tested in 113 patients, 69 mutations occurred in 25 genes. Among them, 105 patients (92.9%) carried at least 1 gene mutation, and the median number of mutations was 2 (1-5). Fifty-one patients were diagnosed with myelodysplastic syndrome (MDS), and the related mutant genes were detected; 62 patients were diagnosed with atypical aplastic anemia or cytopenia of unknown significance. The total effective rate of treatment for 113 patients was 65.5% (74/113).Conclusion:The next-generation sequencing helps to diagnose patients with unexplained pancytopenia, and provides targeted strategies for finding new treatments and prolonging the overall survival of patients.
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Objective To provide useful reference for the pre-operative design and operation by 3D reconstruction and simulation techniques in rhinoplasty.Methods From October 2016 to March 2017,the laser scanning and 3D reconstruction were performed on 25 patients before operations.The facial morphology and the simulated effect were ontained.All patients were female and they were 18 to 39 years old.The average age was 26 years old.The outline and form were normal.We discussed with patients to confirm the ideal nasal 3D simulated effect.In the process of operation,we were guided by the data of soft measures and 3D printing model.Results Among 25 patients who werefollowed up postoperatively.We got intuitive,accurate 3D reconstruction graphs and simulated surgery procedures in all cases.Being applied to actual surgery procedure,23 patients were satisfactory with postoperational results.Two patient's nasal tip dropped.We were satisfied with the results after repair.Conclusions 3D reconstruction and simulation techniques improve surgical precision and operation planning selection,which is a good aided method in rhinoplasty.
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We investigated the cellulase-assisted extraction and anti-ultraviolet activity of water-soluble polysaccharides from the root of Flammulina velutipes on Caenorhabditis elegans. A Box-Behnken design experiment with three factors and three levels, including enzymolysis temperature, microwave time, and microwave power, was designed on the basis of the results of single-factor experiments. For improving the polysaccharide yield of F. velutipes root, the following optimal extraction conditions were used: 52.67 degree C enzymolysis temperature, 80s microwave time, and 144 W microwave power. Under optimal conditions, the actual measured value of the yield was 2.01% [w/w] and the predicted value was 2.06% [w/w]. One fraction [FRP-2] was isolated and purified, and its characteristics were analyzed. The average mean molecular weight of FRP-2 was measured to be 2.60×105 Da, and its monosaccharide composition is mainly glucose. The sugar units are present both in the alpha-configuration and beta-configuration. Moreover, FRP-2 exhibited certain antiultraviolet activity to C. elegans when the polysaccharide concentration ranged between 0.05mg/mL and 0.20mg/mL
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Objective To establish the in vitro biofilm model of Laribacter hongkongensis(LH),to analyze the type Ⅰ integron related genes carried by LH and to investigate the mechanism of LH resistance to quinolones.Methods The biofilm forming abilities of LH clinical isolates were determined by Giemsa staining qualitative method and by crystal violet staining semi-quantitative method.The sensitivity of LH to norfloxacin,ofloxacin,levofloxacin,ciprofloxacin and lomefloxacin in both planktonic and biofilm conditions were dectermined by broth microdilution susceptibility tests.Type I integron related genes carried in 18 LH strains resistant to quinolone were detected by PCR amplification method.Results The detection results by Giemsa staining demonstrated that 36 strains in 55 LH clinical isolates formed visible biofilm,and the biofilm formation rate was 65.4%(36/55).In the biofilm forming ability detected by crystal violet staining semi-quantitative method,OD560≤0.15 was in 8 strains of LH,0.150.20 in 7 strains respectively.The levels of minimal biofilm inhibitory concentration in norfloxacin,ofloxacin,levofloxacin,ciprofloxacin and lomefloxacin to LH were respectively higher than the minimal inhibitory concentration(MIC) in the corresponding floating bacteria(P<0.05).Among 55 strains of LH,18 strains were resistant to quinolones,the resistance rate was 32.7%,and the type I integron in 18 strains of LH carried the drug resistant genes,these drug resistant genes played the drug resistance role in corresponding bacterial strains.Conclusion Drug resistance gene formation and widespread of LH may be associated with type I integron.
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Objective To investigate the effect of different dose of atorvastatin on endothelium dependent vasodilatation function in hypertensives without hyperlipemia in attempt to verify the hypothesis of "beyond antihyperlipimia" effect of statins.Methods Fifty-five hypertensives without hyperlipemia were randomly to receive atorvastatin(10 mg/d,n=25)or(20 mg/d,n=30).Twenty-five normotensives were enrolled in control group.Serum cholesterol were determined.Flow-mediated dilation(FMD)and endothelium-independent dilatation(EID)were measured with high-resolution ultrosonography before and after 4 weeks atorvastatin.Results Compared with control group,FMD were significant decreased in hypertensives without hyperlipemia.FMD were improved after atorvastatin for 4 weeks(atorvastatin 10 mg group:7.5%?2.7% vs 11.5%?3.1%,P
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Objective To evaluate the effect of superficial temporal fascia flap for the reconstruction of temporal depression. Methods According to the degree of temporal depression, we applied folded superficial temporal fascia to reconstruct the temporal depression, combined with forehead and orbital subperiosteal facelift. Results Postoperative follow-up for 1~2 years showed that the method used in subperiosteal facelift combined with temporal depression, maintained a satisfactory long-term full contour. And it improved the facial contour.Conclusions Application of superficial temporal fascia flap in reconstructing the temporal depression combined with facelift for one-stage is a good method. It can get the purposes of tightening the skin and improving the facial contour together. Good results are achieved.